Currently there are no citations for Ponceau S Staining Solution. Do you know of a great paper that uses Ponceau S Staining Solution from Tocris? apply to Products provided by CST, its affiliates or its distributors. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. Access advice and support for any research roadblock, Full event breakdown with abstracts, speakers, registration and more, Explore the power of knock-out cell lines for your research. Ponceau S Staining Solution Support Products, View other related Ponceau S Staining Solution Support Products, Sander H, Wallace S, Plouse R et al. Stain used to detect protein bands after Western blotting, 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt, OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1, Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution). NOT FOR USE IN DIAGNOSTIC PROCEDURES" For licensing inquiries, please contact [email protected]. All transferred protein slowly becomes visible as red bands on the membrane, with highest intensity of staining reached within 5 minutes incubation with Ponceau S. Rinsing the membrane with water after Ponceau S staining can help reduce background staining on the blotting membrane. Changing to another country might result in loss of shopping cart. It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections. 6. Constituents: 0.1% Ponceau S, 0.96% Acetic acid. This ponceau S solution was used to stain total protein that had been applied to PVDF membrane using during a slot blot experiment. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). services used by Customer in connection with the Products. Store at Room Temperature. 3. Ponceau red stain is not stable and will disappear if you destain longer.3. As little as 250 ng protein can be rapidly visualized with Ponceau S stain. reviews that match your criteria. Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services. 1. For batch specific data refer to the Certificate of Analysis. Note: Mouseover a species abbreviation on the product page to display the fullname. By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. Any use of Product for diagnostic, To get a clear background, wash the membrane quickly in PBS after staining2. Protein samples were resolved in NuPAGE 412% Bis-Tris gel (Invitrogen) and transferred to 0.2 m PVDF membrane. Following staining, membranes can be de-stained with any buffer of pH >7 or by transfer of the membrane into blocking buffer (e.g. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1). Get the latest news, product updates, and promotions: Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution). Chemical Name: 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt. Tocris products are intended for laboratory research use only, unless stated otherwise. All rights reserved. Alternatively, a photograph of the stained membrane can also be taken. All Rights Reserved. documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or To get a clear background, wash the membrane quickly in PBS after staining2. any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any Tocris products are intended for laboratory research use only, unless stated otherwise. The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection. Ponceau S Solution (ab270042) is a ready-to-use reversible stain which allows you to check the efficiency of electrophoretic transfer of SDS-denatured proteins to nitrocellulose and PVDF membranes. No product specific FAQs exist for this product, however you may. Publishing research using ab270042? Tocris Bioscience is the leading supplier of novel and exclusive The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your This enables one to incubate the different membrane sections with various primary antibodies. TBS/BSA). There are no specific FAQs related to this product. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. Simply enter the mass of reagent and the target concentration and the calculator will determine the The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1). It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). copyright notices or markings, (d) use the Products solely in accordance with Please let us know. We use Ponceau S (Cat. Minneapolis, MN 55413 Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. Wash membrane in 1X TBS-T several times for 5 min at room temperature until protein bands are no longer visible. Wash membrane in ddH2O until distinct reddish-pink protein bands are visible (1-5 min). commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying Your The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. This enables one to incubate the different membrane sections with various primary antibodies. This product is manufactured by Expedeon, an Abcam company. Copyright 2022 Bio-Techne. life science research with over 30 years' experience in the Mar 23 2019 [PMID: 30914243] (WB), Review with no image -- $10/7/6/$10 CAD/70 Yuan/1110 Yen, Review with an image -- $25/18/15/$25 CAD/150 Yuan/2500 Yen. FAX 612 656 4400. Keywords: Ponceau S Staining Solution, Ponceau S Staining Solution supplier, Ponceau, S, Staining, Solution, stain, detect, protein, bands, western, blotting, Labels, and, Stains, 5225, Tocris Bioscience. There are currently no reviews for this product. 2022 Novus Biologicals, All Rights Reserved. 30 ug of protein was loaded per well and image was taken using a LiCor Odyssey imager The staining worked but the signal was not as strong as other Ponceau S stains I have used before. The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection. Protein samples were resolved in NuPAGE 412% Bis-Tris gel (Invitrogen) and transferred to 0.2 m PVDF membrane. Calculate the mass, volume, or concentration required for a solution. We invite you to request* your copy today! If desired, mark proteins with waterproof ink or a pencil. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. No. structure or technology of the Products, or use the Products for the purpose of developing any products or services that would Please contact us to place your order, or try again later. Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms View all citations for the Staining Kits and Reagents platform. Citations are publications that use Tocris products. Be the first to Please let us know so that we can cite the reference in this datasheet. Be the first to review our Ponceau S Staining Solution and receive a gift card or discount. Calculate the dilution required to prepare a stock solution. This product is stable for 12 months. Your browser does not have JavaScript enabled and some parts of this website will not work without it. compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or If you know of a relevant reference for Ponceau S Staining Solution, please let us know. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available Take a look at our BETA site and see what weve done so far. *When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online). Purchase these through your usual distributor. Bio-Techne uses cookies to provide you with a great website experience. Expedeon product code 5999-1L is the same as the1000 mL size of this product. The technical data provided above is for guidance only. CST Product Terms of Sale and any applicable Visit our, Salinovich, O. and Montelaro, R.C. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs. Azide-containing monosaccharide building block; used as glycoprotein, glycolipid and glycoRNA probe, Amine reactive biotinylation reagent, cell permeable. Lanes 1 to 8 Thermo Scientific Page Ruler Plus, lane 2 E.coli lysate, lane 3 Chicken lysate, lane 4 2 g BSA, lane 5 1 g BSA, lane 6 500 ng BSA, lane 7 250 ng BSA. If incorrect, please enter your country/region into the box below, to view site information related to your country/region. For batch specific data refer to the Certificate of Analysis. You can now submit reviews for your favorite Tocris products. To download a Certificate of Analysis, please select a batch below. $25/18/15/$25CAN/75 Yuan/1250 Yen for a review with an image, $10/7/6/$10 CAD/70 Yuan/1110 Yen for a review without an image. Ponceau S Staining Solution is a stain used to detect protein bands after Western blotting. review today?! Following protein electrotransfer, rinse membrane briefly in ddH2O to remove any detergent that may inhibit staining. Our guide highlights the use of Fluorescent Probes and Dyes in research and provides background information on various imaging techniques. Shipped at Room Temperature. Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. ab270042 has not yet been referenced specifically in any publications. review Ponceau S Staining Solution and earn rewards! industry. The technical data provided above is for guidance only. It was previously called Ponceau S Solution - 1L. Ponceau S waste: Ponceau S staining for total protein normalization Anal. (1986). By continuing to use this website you acknowledge this and agree to our cookie policy. We offer a 100% guarantee on all our products. decide on the best products for their research. Tocris is a Bio-Techne brand. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments. References are publications that support the biological activity of the product. tools for Get the latest news, product updates, and promotions: Stain to detect protein bands and confirm sucessful transfer prior to antibody incubation.. Best staining for tracking protein transfer in western blot. *When preparing stock solutions always use the batch-specific molecular weight of the product found on 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. the vial label and CoA (available online). vial. There are no reviews for Ponceau S Staining Solution (NB5225). USA, TEL 612 379 2956 Would you like to visit your country specific website? Ponceau red stain is not stable and will disappear if you destain longer.3. For batch specific data refer to the Certificate of Analysis. Biochem. 5. ACD branded products will be available on biotechne.com in the near future. The intensity of staining corresponds to the amount of protein transferred. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. *Please note that Tocris will only send literature to established scientific business / institute addresses. rest. Products sold or licensed by CST Following membrane transfer, users can directly incubate the blotting membrane (PVDF or nitrocellulose) in ~15ml of Ponceau S solution without the need for any wash steps. There are no by the FDA or other regulatory foreign or domestic entity, for any purpose. Copyright 2022 R&D Systems, Inc. All Rights Reserved. Agonists, activators, antagonists and inhibitors, Anti-CYP2C9 antibody [EPR7340] (ab150364). Products in stock will be shipped in 1-3 business days unless specified. Read our general, There are no specific blogs for Ponceau S Staining Solution, but you can. 1. Which Brands are Currently Available on biotechne.com? 2. prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners. review will help other researchers Tocris offers the following scientific literature in this area to showcase our products. The technical data provided above is for guidance only. Any Customer's terms and conditions that are in The reviewer received a reward from Abcams Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions. & Earn an Amazon Gift Card, OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1, Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution), TSA Reagents for Enhancing IHC, ICC & FISH Signals. Customer shall not use any Product for any diagnostic Ponceau S Staining Solution is a stain used to detect protein bands after Western blotting. addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized Guaranteed product quality, expert customer support. ProteinSimple branded instruments are available to quote. Toll-free 800 343 7475 This product is for research use only and is not approved for use in humans or in clinical diagnosis. We have publications tested in 1 application: WB. Incubate membrane in Ponceau S Staining Solution for 5-10 minutes at room temperature. Average Rating: 4.3 (Based on 3 Reviews. 1. Why not submit a Do not dilute or aliquot. Store at room temperature. Support products are. requires a separate license from CST. are provided for Customer as the end-user and solely for research and development uses. Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool, Buffers, Chelators and Reagents Staining Kits and Reagents, View Full Technical Details for Ponceau S Staining Solution. We use Ponceau S (Cat. Please note: All products are "FOR RESEARCH USE ONLY. Proceed with blocking step of Western blot procedure. Fluorescent Probes and Dyes Product Guide, Citations for Ponceau S Staining Solution, GMP & Ancillary Material Grade Small Molecules, Certificate of Analysis / Product Datasheet, Submit a Review representative of CST, are rejected and are of no force or effect. Bacterial peptidoglycan derivative; suitable for 'click'-conjugation to fluorescent dyes. 4. View all citations for the Staining Kits and Reagents platform. Tocris products are intended for laboratory research use only, unless stated otherwise. This product guide provides a background to the use of Fluorescent Probes and Dyes, as well as a comprehensive list of our. ), $50/35/30/$50CAN/300 Yuan/5000 Yen for first to review with an image, $25/18/15/$25CAN/75 Yuan/1250 Yen for a review with an image, $10/7/6/$10 CAD/70 Yuan/1110 Yen for a review without an image. 1998-2022 Abcam plc. Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed No. It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections. Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). Get resources and offers direct to your inbox.
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